Journal: Emerging Microbes & Infections
Article Title: Skin models for cutaneous melioidosis reveal Burkholderia infection dynamics at wound’s edge with inflammasome activation, keratinocyte extrusion and epidermal detachment
doi: 10.1080/22221751.2021.2011621
Figure Lengend Snippet: Keratinocytes utilize pyroptosis as a defence mechanism to eliminate infected cells. (a). Bacterial loads of cells infected at MOI 200, 24 hpi. Extracellular bacteria were eliminated via Imaris 3D surface rendering using Cell Mask signals to define intracellular regions. Bt is displayed in red. Cell periphery and nuclei are labelled via Cell Mask and Hoechst staining and are displayed in green and blue respectively. Scale bar = 100 µm. (b). Area of infection was examined from the confocal micrographs based on bacterial fluorescence intensities. Data presented as mean ± S.D from biological triplicates.
Article Snippet: Extracellular bacteria were excluded via Imaris 3D surface rendering using Cell Mask signals to define intracellular regions.
Techniques: Infection, Bacteria, Staining, Fluorescence